fig3

Figure 3. Normalization of diastolic SR Ca²⁺ release events due to genome editing. (A) Representative confocal line scan images showing sarcoplasmic reticulum (SR) Ca²⁺ sparks in ventricular myocytes isolated from R176Q/+ mice treated with control (Con) AAV9 or gRNA-SaCas9, or WT littermate mice. (B) Quantification of Ca²⁺ spark frequency (CaSpF), (C) SR Ca²⁺ leak measured using the caffeine dump protocol, and (D) ratio of Ca²⁺ spark frequency and SR Ca²⁺ load. Numbers of cells and mice are indicated. P-values are based on the nested one-way ANOVA test.