fig5

Figure 5. Senolytic D+Q treatment abrogates the SASP. (A) Secretion of SASP proteins, IL-6, IL-8, CCL11, CXCL5, CXCL1, CCL7, in media of iPSC-CMs cultured alone for 7 days (CTRL 7d), co-cultured with senCPCs for 7 days (Co-culture 7d), cultured alone for 17 days (CTRL 17d), co-cultured with senCPCs for 17 days (Co-culture 17d), or co-cultured with senCPCs and treated with D+Q (Co-culture D+Q 17d). Data are Mean ± SD. Significance was determined by ANOVA followed by Tukey’s post-hoc test to identify the differences. *P < 0.05 vs. 7d and 17d CTRL; ^†P < 0.05 vs. 7d and 17d co-culture. Individual data points represent independent replicates/wells. (B) Secretion of SASP proteins, IL-6, IL-8 and CXCL1, in medium of HUVECs cultured alone for 7 days (CTRL 7d), co-cultured with senHUVECs for 7 days (Co-culture 7d), cultured alone for 17 days (CTRL 17d), co-cultured with senHUVECs for 17 days (Co-culture 17d), or co-cultured with senHUVECs and treated with D+Q (Co-culture D+Q 17d). Data are Mean ± SD. Significance was determined by ANOVA followed by Tukey’s post-hoc test to identify the differences. *P < 0.05 vs. 7d and 17d CTRL; **P < 0.05 vs. 17d CTRL; ^†P < 0.05 vs. 7d and 17d co-culture. Individual data points represent independent replicates/wells.