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Figure 1. Co-culture with senCPCs decreases number of iPSC-CMs and their cell cycle activity. (A and B) iPSC-CM crystal violet staining (A) and BrdU-positive staining (B) when cultured alone (CTRL 7d) or co-cultured with senCPCs (Co-culture 7d). Data are Mean ± SD. Significance was determined by Student’s t-test, *P < 0.05, ** P < 0.01. Individual data points represent independent replicates/wells. Representative field of view micrograph images of iPSC-CMs stained with crystal violet (A). Scale bar = 200 μm. Representative field of view micrograph images of iPSC-CMs labelled and immunostained with BrdU (green) and nuclei counterstained with DAPI (blue) (B). Scale bar = 50 μm. (C and D) SA-β-gal activity (C) and p21 expression (D) of iPSC-CMs when cultured alone (CTRL 7d) or co-cultured with senCPCs (Co-culture 7d). Data are Mean ± SD. Individual data points represent independent replicates/wells. Representative fields of view micrograph images of iPSC-CMs stained by enzymatic SA-β-gal assay (blue, upper panel) and nuclei counterstained with DAPI (blue, lower panel) (C). Scale bar = 100 μm. Representative fields of view micrograph images of iPSC-CMs immunostained for p21 (red) and nuclei counterstained with DAPI (blue) (D). Scale bar = 50 μm.