fig2

Figure 2. Genome-wide RNA sequencing analysis of aortas from young and old HCR and LCR rats. (A) Heat map representation of unsupervised hierarchical clustering analysis with top 1000 most variable genes. (B) Principal component analysis of the whole transcriptome from young and old HCR and LCR aortas. (C) Venn diagram of differentially expressed genes across four comparisons between young and old HCR and LCR aortas. (D) Volcano plot of differentially expressed genes between old HCR and LCR with FDR ≥ 0.05 and fold change ±1.5. Gene ontology pathway enrichment analysis showing top selected KEGG signaling pathways upregulated (E) or downregulated (F) in old HCR compared with old LCR aortas.