fig3

Figure 3. Differentially expressed genes (DEGs) in cardiac myocytes upon cWNT/β-catenin activation or suppression. (A) Volcano plot of DEGs showing 238 upregulated (red), 131 downregulated (blue) and 12,246 unchanged (black) genes upon suppression of the β-catenin in the cardiac myocytes compared to the wild type myocytes. (B) Volcano plot of the DEGs in the Myh6-Mcm:Ctnnb1^GoF cardiac myocytes compared to the wild-type myocytes. The upregulated DEGs are shown in red (1050), the downregulated in blue (641), and the unchanged genes in black (9397). (C) Volcano plot of the DEGs comparing the transcripts in the Myh6-Mcm:Ctnnb1^GoF and Myh6-Mcm:Ctnnb1^LoF myocytes showing 731 upregulated genes in red, 345 downregulated genes in blue, and 12,266 unchanged genes in black in the postmitotic cardiac myocytes. (D-F) Heat map of the DEGs between wild type vs. Myh6-Mcm:Ctnnb1^LoF (D), between wild type vs. Myh6-Mcm:Ctnnb1^GoF (E), and between Myh6-Mcm:Ctnnb1^GoF vs. Myh6-Mcm:Ctnnb1^LoF (F), which show distinct genotype-dependent clustering. (G) GSEA analysis of the DEGs in the Myh6-Mcm:Ctnnb1^LoF cardiac myocytes showing upregulated genes enriched in the oxidative phosphorylation (OXPHOS) pathway and downregulation of genes involved in the EMT pathway. (H) GSEA analysis of the DEGs depicting upregulated genes as being targets of E2F and mapped to the G2/M cell cycle checkpoint in the β-catenin activated cardiac myocytes. (I) GSEA analysis of the DEGs comparing the β-catenin activated and suppressed cardiac myocytes showing upregulated genes are enriched for the E2F targets and those involved in the G2/M checkpoint.