fig6

Figure 6. Ulk1S913A knock-in (KI) inhibits autophagy in the heart. Ulk1S913A KI and wild type (WT) mice were euthanized at six months of age. (A) Immunoblotting of LC3, p62, and α-tubulin in Ulk1S913A KI mouse hearts at baseline and 4 h after chloroquine (CQ) treatment. (B) The relative band density of LC3-II. The LC3-II/Tubulin in the WT control (Ctrl) group were designated as 1. *P < 0.05 vs. WT-Ctrl; ^#P < 0.05 vs. WT-CQ. (C) The relative band density of p62. The p62/Tubulin in the WT-Ctrl group were designated as 1. *P < 0.05 vs. WT-Ctrl; ^$P < 0.05 vs. S913A-Ctrl. (D) A scheme summarizing the major findings of the study.