fig5

Figure 5. Hearts injected with a combination of STEMIN and YAP5SA revealed little if any staining for immune cells infiltrates. (A) Anti-F4/80 antibody recognizes the mouse F4/80 antigen, a 160 kD glycoprotein expressed by murine macrophages^[20]. The secondary antibody (goat anti-rat Alexa Fluor 488) showed staining overlapped the infarcted ventricular wall of the control hearts. Anti-Ly6g stained a marker expressed predominantly on neutrophils^[21]; in addition, a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells showed staining in the infarcted heart. (B) Sectioned STEMIN and YAP5SA synthetic mmRNA treated infarcted hearts after four weeks did not reveal fibrotic scarring or staining with anti-F4/80 or anti-Ly6g.