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Figure 4. Injections of the combination of STEMIN and YAP5SA mmRNA repaired infarcted adult mouse hearts in vivo. (A) Schematic diagram of long-term in vivo experiments in adult mice (100 days old) infarcted by LAD ligation. Five equal injections of STEMIN (50 µg in 38 µL) and YAP5SA (50 µg in 22 µL) with 33 µL Lipofectamine MessengerMAX around the cardiac infarct. In addition, Individual synthetic mmRNA were tested for their ability to restore cardiac function following infarctions. The control group was injected with PBS (60 µL) with 33 µL Lipofectamine MessengerMAX. Echo Doppler measurements were taken prior to infarction and one, two, and four weeks after mmRNA injections. (B) Cardiac function was graphed for Echo Doppler measurements of the infarcted controls (six mice) and the combination of STEMIN and YAP5SA mmRNA experimental group (four mice) from the same inbred liter. The EF declined greater for the control than the combination experimental group by the second and fourth weeks, and both were significantly different
