fig3

Figure 3. Injected STEMIN and YAP5SA mmRNA induced cell replication and DNA replisome factors. (A) In a second injected heart, anti-phospho-histone H3 stain of a serial section was observed along two needle tracts marked by white arrows. Robust EdU stain overlap anti-pHH3 stains exactly in the same orientation and location. A key component of the DNA replisome was stained by anti-CLASPIN overlapped anti-pHH3 and Edu stains. Anti-NANOG stained NANOG was observed, but peaked levels were probably about 12 h earlier^[15]. (B) In a third injected heart, anti-ORC2 (Origin recognition complex subunit 2) and anti-MCM2 showed the stained markers of the pre-initiation phase in early G1 stage induced in the injected hearts. Anti-ATR stained Ataxia telangiectasia and Rad3-related, an essential kinase that is active in S phase which overlapped other DNA replisome factors. Thus, STEMIN and YAP5SA mmRNA treatment fostered cell cycle entry by promoting DNA replication in the G1 phase.