fig7

Figure 7. Cardiomyocytes transfected with a combination of SRF153(A3) and YAP5SA mmRNA mutants showed a primitive genetic expression profile. (A) Schematic diagram of NRVM transfected with SRF153(A3) and YAP5SA mmRNA mutants individually and in combination with optimized Lipofectamine MessengerMAX. RNA was collected for RNA sequencing. (B) Heat map of cytokinesis (GO:0000910) genes generated from RNA-seq data at 32 h after transfection. (C) Heat map of spindle assembly (GO: 0051225) genes generated from RNA-seq data 40 h after transfection. (D) Heat map of cardiac muscle cell differentiation (GO:0055007) genes generated from RNA-seq data 48 h after transfection. (E) GSEA plot for differentially regulated genes for DNA replication and cell cycle [NES (normalized enrichment score, relative to control) and FDR q-value (after FDR correction)]. (F) GO-term analysis of upregulated DEG genes in 48 h post-transfection combination group versus the control group (top). GO-term analysis of downregulated DEG genes in 48 h post-transfection combination group versus the control group (bottom).